A city and historical diocese in Italy. The diocese was originally under the immediate jurisdiction of the Holy See and comprised seven towns of the Province of Rome. The Diocese of Acquapendente was under the ecclesiastical jurisdiction of Orvieto until 1649. That year, in consequence of a conspiracy, Cristoforo Girarda, a Barnabite of Novara, Bishop of Castro, was assassinated. In punishment of this crime, Innocent X ordered Castro to be destroyed, and raised Acquapendente to the dignity of an episcopal city (Bull, 13 September, 1649). Its bishops, however, retain the appellation "post Castrenses." The first incumbent of the new See was the Hieronymite (il gerosolimitano) Pompeo Mignucci of Offida, who had been Archbishop of Ragusa. He took possession 10 January, 1650. The diocese was united with the dioceses of Bagnoregio, Montefiascone and Viterbo e Tuscania to form the Diocese of Viterbo, Acquapendente, Bagnoregio, Montefiascone, Tuscania e San Martino al Monte Cimino ("Diocese of Viterbo")
Acquapendente was also the birthplace of the anatomist Hieronymus Fabricius.
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